Abstract
Excessive use of pesticides to satisfy the demand of a rapidly increasing population has posed numerous problems for human health and the environment. Therefore, we developed a nanocomposite comprised of rice husk-derived dialdehyde nanocellulose capped silver nanoparticles (AgNP@DANC) as an efficient immobilizing material for acetylcholinesterase (AChE) to develop a novel biosensor. This revolutionary enzyme-based biosensor was used for the rapid detection of organophosphate pesticides (OPs) using chlorpyrifos (CPF) and malathion (MLT) as representative pesticides in food stuffs. The detection of OPs is based on the inhibition of the catalytic activity of AChE in the presence of CPF and MLT coupled with the aggregation of silver nanoparticles (AgNPs) due to thiocholine that can be analyzed for rapid screening. The developed biosensor for CPF and MLT shows ultrasensitive behaviour over a linear range of 1 × 10−3 to 1 × 10-19 M and 1 × 10−3 to 1 × 10−17 M, respectively. Further, the sensitivity of the developed biosensor was also studied for real spiked samples. The developed biosensor shows great potential and sensitivity; thus, it could be a promising biosensor for the rapid and on-site detection of many OPs.
Published Version
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