Development of compartmentalized innervation of the rat gluteus maximus muscle

Abstract

The innervation territories of the nerves to the gluteus maximus muscle were investigated in neonatal rats by using evoked electromyographic mapping techniques. Similar methods were employed to determine the terminal fields of individual motoneurons. Both at birth and after the period of neuromuscular synapse elimination, rat gluteus maximus is partitioned by its two muscle nerves. Each of these partitions is further divided into neuromuscular compartments by the primary branches of the muscle nerves. The terminal fields of individual motoneurons are about as well localized in early neonates as in 26 day-old animals. Intracellular records from gluteal muscle cells reveal that some myotubes in a narrow region near the center of the muscle receive inputs from both of the muscle nerves during the period of polyneuronal innervation. Such cross-compartmental innervation could be demonstrated only until the eighth postnatal day, even though polyneuronally innervated muscle cells were found as late as postnatal day 18. Analysis of the trajectories of individual axons filled with horseradish peroxidase indicates that they branch into only one primary branch of the nerves to gluteus maximus, both in newborn and 14 day-old pups. The innervation of gluteus maximus at birth is essentially restricted to neuromuscular compartments. Synapse elimination probably plays a minor role in establishing neuromuscular compartments.