Abstract
A rapid, sensitive immunochromatographic (IC) assay was developed to detect ricin toxin in assay buffer and food samples. The assay was based on the sandwich format using a monoclonal antibody (mAb) and a polyclonal antibody (pAb) of two distinct specificities. The ricin-containing sample was added to the membrane and allowed to react with pAb-coated particles. The mixture was then passed along the porous membrane by capillary action past the mAb in the detection zone, which would bind the particles that had ricin bound to their surfaces, giving a red colour within the detection zone with an intensity proportional to the ricin concentration. In the absence of ricin, no immunogold was bound to the solid-phase antibody. With this method, 30 ng/ml of ricin toxin was detected in less than 10 min, and the assay also showed no cross-reactivity with abrin toxin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
