Development of biosensor for protein tyrosine kinases

Abstract

Protein tyrosine kinase plays an important role in many cellular events, and many diseases are found to be associated with the dysregulation of various protein tyrosine kinases. However, the conventional methods for the detection of the activity of tyrosine kinase were expensive, specific instrument required, and high risk of radioactive contamination. In this study, A simple, quick and label‐free detection method was developed and developed. The general concept of this detection method is based on the fact that tyrosinase‐based amperometric biosensor can catalyze an electrochemical response of tyrosine but not its phosphorylation form, phosphor‐tyrosine. Further studies showed that the tyrosinase‐based biosensor could recognize tyrosyl residue within either dipeptides or heptapeptide p60c‐src substrate I, a specific peptide substrate for Src kinase. The linear dynamic range of detection to p60c‐src substrate I was studied with a sensitivity of 0.71μA μM‐1cm‐2. The effect of various kinase inhibitors in the Src activity was also studied.Financially supporting by NSC‐97‐2311‐B‐009‐001 MY3.