Development of biological oxygen demand biosensor for monitoring the fermentation industry effluent.

Neelam Verma,Ashish Kumar Singh

doi:10.5402/2013/236062

Abstract

A biosensor was developed for the determination of BOD value of fermentation industry effluent. The developed biosensor was fabricated by immobilizing the microbial consortium on cellulose acetate (CA) membrane in close proximity to a DO probe electrode. The microbial consortium was harvested from the fermentation industry effluent. The BOD biosensor was calibrated by using a solution containing the equivalent amount of glucose/glutamic acid (GGA) as a standard sample solution. The response time was optimized by immobilizing different concentrations of cell biomass on CA membrane. Once the response time was optimized, it was used for determination of BOD of fermentation industry effluent. For analysis of fermentation industry effluent, the response time was observed 7 minutes with detection limit 1 mg/L. Good linear range with GGA standard solution was observed, R 2 0.99 with relative standard deviation (RSD) <%. The observed BOD value by biosensor showed a good comparison with the conventional method for the determination of BOD.

Highlights

IntroductionBOD5 which e United States includes Biochemical oxygen demand (BOD) effluent limitations in its secondary treatment regulations
Biochemical oxygen demand (BOD) is one of the most important and widely used parameters for characterizing the organic pollution of water and wastewater, which is estimated by determining the amount of oxygen required by aerobic microorganisms for degrading organic matters in wastewater.Conventional BOD method is the well-known needs 5-day incubation at 20∘C in the dark [1].BOD5 which e United States includes BOD effluent limitations in its secondary treatment regulations
We propose a new analytical approach that has very cost-effective and simple idea for determination of BOD value of fermentation industries effluents. is new approach utilizes microbial consortium that was isolated from fermentation industry effluent and separately immobilized on the cellulose acetate membrane. e primary objective was isolation of microbial consortium, immobilization on transducer, that is, DO probe, optimization of response time, and comparing BOD by using this biosensor with Winkler’s method (BOD5)

Summary

Introduction

BOD5 which e United States includes BOD effluent limitations in its secondary treatment regulations. Secondary sewage treatment is generally expected to remove 85 percent of the BOD measured in sewage and produce effluent BOD concentrations with a 30-day average of less than 30 mg/L and a 7-day average of less than 45 mg/L. Most pristine rivers will have a ve day carbonaceous BOD below 1 mg/L. Polluted rivers may have a BOD value in the range of 2 to 8 mg/L. Municipal sewage that is efficiently treated by a three-stage process would have a value of about 20 mg/L or less. E generally lower values in the US derive from the much greater water use per capita than in other parts of the world [2] Untreated sewage varies but averages around 600 mg/L in Europe and as low as 200 mg/L in the US, or where there is severe groundwater or surface water in ltration. e generally lower values in the US derive from the much greater water use per capita than in other parts of the world [2]

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Conclusion