Development of biocatalytic microbial ecosystem (FPUS@RODMs@In-PAOREs) for rapid and sustainable degradation of various refractory organics

Abstract

The removal of diverse refractory organics from complex industrial wastewater continues to be a challenge. Although biological treatments are commonly employed, only partial degradation and increasing emergence of nitrogenous compounds, i.e., nitrate (NO3) and nitrite (NO2) would pose severe toxicity to the intact microbes. Herein, an efficient biocatalytic microbial ecosystem (BCME) was designed over a porous bio-carrier made of a functional polyurethane sponge (FPUS). The BCME comprised a unique set of organisms (RODMs) with novel metabolism, efficiently degrading highly-concentrated aromatics. Strategic enzyme immobilization was utilized to introduce in-situ production and aggregation of the oxidation and reduction enzymes (In-PAOREs) onto the FPUS, thereby ensuing sustained functions of the RODMs community. The developed FPUS@RODMs@In-PAOREs system was found to enhance the refractory organics removal rate to 4 kg/m3/day, and it would be attributed to the enzymatic catalysis of refractory organics (2000 mg/L) accompanied by the removal of COD (1200 mg/L) and nitrogenous compounds (200 mg/L). Besides, the fluctuating concentration of extra polymeric substances (EPS) played a dual role through enhancing adhesion, promoting the development of a functional microbial ecosystem, and creating an EPS gradient within the FPUS bio-carrier. This differential distribution of enzymes was established to significantly boost biocatalysis activity reaching 400 U/g VSS.