Abstract
Laboratory and glasshouse bioassays were developed in Australia and the US for evaluating wild pea ( Pisum fulvum Sibth. & Sm.) accessions for resistance to pea weevil ( Bruchus pisorum) (L.), one of the most important pests of cultivated pea ( Pisum sativum L.) in Europe, India, North and South America and Australia. A field experiment and laboratory tests showed that flat and swollen P. sativum pods and pods longer than 10–20 mm in length provide optimal or near optimal oviposition substrates. Dual-choice and no-choice laboratory bioassays were then developed using excised flat and swollen pods 30–40 mm in length to screen P. fulvum accessions for pea weevil resistance. A glasshouse experiment to measure antibiosis resistance in seed demonstrated the effectiveness of manually transferring weevil eggs to intact pods of different maturity stages on potted P. fulvum plants. The laboratory dual-choice and no-choice tests and the glasshouse bioassays are complementary and fulfill the requirement for a reliable screening method to identify the resistance status of P. fulvum germplasm to B. pisorum.
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