Development of Automatic Stimulating Cell System Using Mechanical Vertical Force

Abstract

This study developed an automatic monitoring system with a vertical mechanical force to stimulate cancerous cartilage sw1353 cells. The system is controlled by the human-machine interface to stretch cell-cultured substrate continuously which the cells cultured on the PDMS substrate. This system can precisely control the substrate strain, stretch frequency, stretch interval and stopping time when the substrate is stretched. The automatic monitor system can stimulate the cancerous cartilage cells which cultured on the PDMS. In addition, the electrophopresis force was used to measure the cell adhesive ability for the stretched and unstretched cells. The automatic monitoring system with a vertical mechanical force stimulator can stretch substrate which is stretched the strain capacity of substrate ranges from 0.5 to 9%.The stretching frequency ranges from 0.4 to 2.4Hz for this system. The average error of system ranges from 0.033 to 0.034mm after it has conducted 30 consecutive movements. When the cells were conducted 3% substrate strain, 0.8Hz, and 30-minute continuous cycle of tensile strength, the adhesion force of cell ranges from 5.57 to 7.93nN, significantly higher than the unstretched cell which range from 0.92 to 1.25nN.The experimental results showed that the stretching stimulation highly correlates to the cell adhesion.