Abstract
A continuous-flow biosensor arrangement based on the dual immobilization of a biocatalyst on controlled-pore glass and the reaction product on a resin support both packed in the flow-cell of a photometric detector for the determination of sulphite is proposed. The method thus developed affords low detection and quantitation limits (3 and 10 ng ml −1 of sulphite, calculated as ±3 σ and ±10 σ, respectively, for n = 20), wide linear range (10–1000 ng ml −1 of sulphite), good precision (R.S.D. = 2.6% for 60 ng ml −1 and 0.7% for 200 ng ml −1 of the analyte), and a sampling frequency of 16 h −1. The hydrodynamic assembly has been applied to the determination and recovery of the analyte in spiked, aqueous samples with excellent results.
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