Development of an oligonucleotide-based microarray for the detection of foodborne viruses

Abstract

Foodborne viral diseases are a major public health problem that poses significant social and economic strains. Human astrovirus (AstV), norovirus (NoV), human rotavirus (RoV), and hepatitis A virus (HAV) are frequently reported foodborne pathogens. The microarray is a molecular diagnostic method that is highly specific and widely used due to its plethora of applications in microbiology. This investigation characterized the development of concurrent diagnostic procedures for AstV, NoV, RoV, and HAV. We employed microarray techniques using specific oligonucleotide probes designed for each target virus and analyzed fluorescent signals by hybridization reaction on chips. The selected oligonucleotide probes yielded strong hybridization signals for target viruses without non-specific reactions to other viruses. The developed microarray platform represents a promising means for detection of a broad range of pathogens and, therefore, could improve the diagnosis of foodborne viruses.