Abstract
Conventional schemes used for identification of Salmonella involve both biochemical and serological testing which may require up to a week to perform, especially when identifying atypical strains. We report here a simple indirect whole cell ELISA test which uses a murine hybridoma monoclonal antibody M105 for the rapid identification of Salmonella. Two hundred and eight strains of 89 different serotypes of Salmonella from 6 different subspecies involving 26 O serogroups were all correctly identified by the ELISA test. Three Citrobacter freundii and four Escherichia coli isolates submitted to the National Laboratory for Enteric Pathogens as suspected Salmonella cultures tested negative by indirect whole cell ELISA.
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