DEVELOPMENT OF AN IN VITRO MULTIPLICATION SYSTEM FOR RUBUS LOGANOBACCUS L.

Abstract

The Rubus loganobaccus L. variety is a hybrid produced from a cross between Rubus idaeus and Rubus ursinus, which is well-known for its exatic distinguished fruits. However, a commercial cost-effective multiplication method has not been introduced yet, which micropropagation as a reliable technique could be an efficient economic procedure to generate uniform plant materials. In this case, an experiment carried out using plant buds explants the most appropriate disinfection treatments found out to be 1 to 5 min in 0.1% mercuric chloride in which healthy buds grew with no fungal/bacterial contamination. The highest rooting percentage (96.66%) and the lowest rooting percentage (60%) were observed in concentrations of phytohormones in MS +BA( 1 mg∙L-1) IBA + (0.1 mg∙L-1) and MS + 2ip (1 mg∙L-1) + IBA (0.1 mg∙L-1), respectively. The maximum and the minimum average root length were 3 cm and 1 cm, respectively. In the acclimatization stage of the plant root, the maximum percentage of survived and fresh pots 96.43%, the maximum average number of branching 8.73, the minimum average number of branching 3.13 in treatments MS + BA( 1 mg∙L-1) + IBA(0.1 mg∙L-1) and hormonefree MS medium, respectively, the maximum branch length 5.22 cm, and the minimum branch length 1.71 cm in MS + 2ip (2 mg∙L-1) + IBA (0.1 mg∙L-1) and AN + BA (1 mg∙L-1) + IBA (0.1 mg∙L-1), respectively, were observed. Overall, this protocol comperhensively addresses the whole process of micropropagation and can be further be used in commercial propagation of Rubus loganobaccus and produce plant materials with superior advantages over conventional methods.