Development of an Electrochemical Biosensor for GFAP with Carbon-Based Substrate for Traumatic Brain Injury

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This study concentrates on creating an electrochemical biosensor designed for the real-time identification and monitoring of biomarkers, specifically Glial Fibrillary Acidis Protein (GFAP). The intended applications involve the diagnosis and prognosis of Traumatic Brain Injury (TBI). TBIs pose a significant global health challenge, impacting millions each year, and existing diagnostic methods, such as neuroimaging, face constraints related to cost, radiation exposure and a greater delay in providing a diagnosis. The suggested biosensor exploits electrochemical techniques, offering benefits such as non-invasiveness and swift results. The primary objective is to advance wearable microelectrodes for TBI detection, with an emphasis on utilizing bodily fluids like sweat as practical samples. Moreover, the biosensor's versatility makes it well-suited for various environments, including extreme conditions.The objective of this research is to enhance the precision of TBI diagnosis and prognosis, thereby ultimately improving the efficacy of point-of-care treatment. Our methodology involves the utilization of the Capture Systematic Evolution of Ligands by Exponential Enrichment (Capture-SELEX) technique to create a highly specific aptamer designed for the detection of Glial Fibrillary Acidic Protein. The biosensor we are developing will feature electrodes modified with aptamers and a carbon-based substrate, enabling continuous real-time monitoring of the target substance. The primary objective of our biosensor is to identify GFAP, thereby reducing risks associated with exposure to radiation, and healthcare costs. To ensure the long-term stability of our biosensor, our design centers around a carbon-based substrate. Carbon-based nanomaterials have garnered significant attention in biosensor applications due to their diverse range of properties, encompassing chemical stability, biocompatibility, functionalization, and biodegradability.