Development of an arginine-based cationic hydrogel platform: Synthesis, characterization and biomedical applications

Abstract

A series of biodegradable and biocompatible cationic hybrid hydrogels was developed from water-soluble arginine-based unsaturated polymer (Arg-AG) and poly(ethylene glycol) diacrylate (PEG-DA) by a photocrosslinking method. The physicochemical, mechanical and biological properties of these hydrogels were intensively examined. The hydrogels were characterized in terms of equilibrium swelling ratio (Qeq), compression modulus and interior morphology. The effects of the chemical structure of the two Arg-AG precursors and the feed ratio of these precursors on the properties of resulting hybrid hydrogels were investigated. The crosslinking density and mechanical strength of the hybrid hydrogels increased with an increase in allylglycine (AG) content in the Arg-AG precursor, as the gelation efficiency (Gf) increased from 80% to 90%, but the swelling and pore size of the hybrid hydrogels decreased as the equilibrium swelling weight (Qeq) decreased from 1890% to 1330% and the pore size from 28 to 22μm. The short-term in vitro biodegradation properties of hydrogels were investigated as a function of Arg-AG chemical structures and enzymes. Hybrid hydrogels showed faster biodegradation in an enzyme solution than in a phosphate-buffered saline solution. Bovine serum albumin and insulin release profiles indicated that this cationic hydrogel system could significantly improve the sustained release of the negatively charged proteins. The cellular response of the hybrid hydrogels was preliminarily evaluated by cell attachment, encapsulation and proliferation tests using live–dead and MTT assay. The results showed that the hybrid hydrogels supported cell attachment well and were nontoxic to the cells.