Arginine-based polyester amide/polysaccharide hydrogels and their biological response

Abstract

An advanced family of biodegradable cationic hybrid hydrogels was designed and fabricated from two precursors via a UV photocrosslinking in an aqueous medium: unsaturated arginine (Arg)-based functional poly(ester amide) (Arg-UPEA) and glycidyl methacrylate chitosan (GMA-chitosan). These Arg-UPEA/GMA-chitosan hybrid hydrogels were characterized in terms of their chemical structure, equilibrium swelling ratio (Qeq), compressive modulus, interior morphology and biodegradation properties. Lysozyme effectively accelerated the biodegradation of the hybrid hydrogels. The mixture of both precursors in an aqueous solution showed near non-cytotoxicity toward porcine aortic valve smooth muscle cells at total concentrations up to 6mgml−1. The live/dead assay data showed that 3T3 fibroblasts were able to attach and grow on the hybrid hydrogel and pure GMA-chitosan hydrogel well. Arg-UPEA/GMA-chitosan hybrid hydrogels activated both TNF-α and NO production by RAW 264.7 macrophages, and the arginase activity was also elevated. The integration of the biodegradable Arg-UPEA into the GMA-chitosan can provide advantages in terms of elevated and balanced NO production and arginase activity that free Arg supplement could not achieve. The hybrid hydrogels may have potential application as a wound healing accelerator.