Abstract
Human enteric viruses such as norovirus (NoV) and hepatitis A virus (HAV) are some of the most important causes of foodborne infections worldwide. Usually, infection via fish consumption is not a concern regarding these viruses, since fish are mainly consumed cooked. However, in the last years, raw fish consumption has become increasingly common, especially involving the use of seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio. Therefore, the risk for viral infection via the consumption of raw fish has also increased. In this study, a virologic screening was performed in 323 fish specimens captured along the Portuguese coast using a tetraplex qPCR optimised for two templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A difference of approximately 1-log was found between the use of plasmid or in vitro transcribed RNA for molecular-based quantifications, showing an underestimation of genome copy-number equivalents using plasmid standard-based curves. Additionally, the presence of NoV genomic RNA in a pool of seabass brains was identified, which was shown to cluster with a major group of human norovirus sequences from genogroup I (GI.1) by phylogenetic analysis. None of the analysed fish revealed the presence of NoV GII or HAV. This result corroborates the hypothesis that enteric viruses circulate in seawater or that fish were contaminated during their transportation/handling, representing a potential risk to humans through raw or undercooked fish consumption.
Highlights
Enteric viruses represent a major risk to human health, being responsible for numerous outbreaks worldwide
The European Centre for Disease Prevention and Control (ECDC), NoV-associated foodborne infections are one of the most frequently reported in the United States and European Union (EU), being, for instance, associated with 457 outbreaks, and, most importantly with 11,125 cases of illness in 2019 (22.5% of total cases) only in the EU [1]
Of all the viruses associated with foodborne infections, NoV and hepatitis A virus (HAV) are the most important viral pathogens regarding the severity of the associated illnesses and their common occurrence worldwide [23]
Summary
Enteric viruses represent a major risk to human health, being responsible for numerous outbreaks worldwide. The European Centre for Disease Prevention and Control (ECDC), NoV-associated foodborne infections are one of the most frequently reported in the United States and European Union (EU), being, for instance, associated with 457 outbreaks, and, most importantly with 11,125 cases of illness in 2019 (22.5% of total cases) only in the EU [1]. NoV and HAV have been estimated to impart high economic losses, mainly associated with the measures taken to reduce their impact on population health [2,3]. States [4], while studies in the Netherlands reported economic losses associated with NoV and HAV to be around EUR 90 million and EUR 2.9 million, respectively [5].
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