Abstract
BackgroundIn this study, we developed a rapid, one step colloid gold strip (CGS) capable of specifically detecting type Asia1 foot-and-mouth disease virus (FMDV). We have produced two monoclonal antibodies (mAb) to type Asia1 FMD (named 1B8 and 5E2). On the test strip, the purified 1B8 labelled with the colloidal gold was used as the detector, and the purified 5E2 and goat anti-mouse antibodies were wrapped onto nitrocellulose (NC) membranes as the test and the control line, respectively. The rapid colloidal gold stereotype diagnostic strip was housed in a plastic case.ResultsIn specificity and sensitivity assay, there was no cross-reaction of the antigen with the other type of FMD and SVDV. The detection sensitivity was found to be as high as 10-5 dilution of Asia1/JSL/05 (1 × 107.2TCID50/50 μL). There was excellent agreement between the results obtained by CGS and reverse indirect hemagglutination assay (RIHA), and the agreement can reach to 98.75%.ConclusionWe developed colloidal gold strips that have good qualities and does not require specialized equipment or technicians. This method provided a feasible, convenient, rapid, and effective for detecting type Asia1 FMDV in the fields.
Highlights
In this study, we developed a rapid, one step colloid gold strip (CGS) capable of detecting type Asia1 foot-and-mouth disease virus (FMDV)
FMDV as pathogeny of foot-andmouth disease (FMD) is a member of the family Picormaviriae and exists in seven immunological distinct serotypes (Asia1, A, O, C, SAT1, SAT2 and SAT3)
Many of sensitive methods such as reverse indirect hemagglutination assay (RIHA) and RT-PCR have been developed to analyze FMDV in nasal swabs, epithelial suspensions and probangs of clinical samples submitted from the field or animals infected experimentally with cell culture [3,4]
Summary
We developed a rapid, one step colloid gold strip (CGS) capable of detecting type Asia foot-and-mouth disease virus (FMDV). The purified 1B8 labelled with the colloidal gold was used as the detector, and the purified 5E2 and goat anti-mouse antibodies were wrapped onto nitrocellulose (NC) membranes as the test and the control line, respectively. During the Aisa type FMD outbreak in the China in 2005, the requirement for a 24 h slaughter policy did not allow sufficient time for laboratory confirmation of suspect infection following clinical diagnosis. Many of sensitive methods such as RIHA and RT-PCR have been developed to analyze FMDV in nasal swabs, epithelial suspensions and probangs of clinical samples submitted from the field or animals infected experimentally with cell culture [3,4]. It is extremely desirable to develop a rapid and convenient detection method for FMDV
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