Abstract
AbstractBACKGROUND: Malachite green (MG) is widely used in fishery, since it is easily adsorbed by fish during waterborne exposure and is rapidly metabolised into leucomalachite green (LMG). However, both MG and LMG are potential carcinogens, teratogens and mutagens. In this study the LMG derivative bearing an amino group on the phenyl ring was synthesised and coupled to carrier proteins. An LMG polyclonal antibody‐based enzyme‐linked immunosorbent assay (ELISA) was developed and characterised.RESULTS: The ELISA standard curve was constructed with concentrations of 0.1–100 ng mL−1. The IC50 value for nine standard curves was in the range 0.9–2.6 ng mL−1 and the limit of detection at a signal‐to‐noise ratio of 3 was 0.02–0.10 ng mL−1. The cross‐reactivity values of the LMG antibody with MG, crystal violet and leucocrystal violet were 95.25, 29.07 and 212.38% respectively, while less than 0.2% cross‐reactivity was found with eight other compounds. For LMG‐spiked water and fish samples, recoveries were 76.2–95.0% and the correlation coefficient of ELISA with high‐performance liquid chromatography (HPLC) was 0.9752 (n = 7). For (LMG + MG)‐spiked fish samples the results of ELISA were similar to those of HPLC.CONCLUSION: The proposed ELISA can be utilised as an analytical tool for detecting the sum of MG and LMG in water and fish muscle samples. Copyright © 2009 Society of Chemical Industry
Published Version
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