Abstract
A simple RP-HPLC method was designed for the quantification of muscimol (5-(aminomethyl)-isoxazol-3-ol) present in five aqueous extracts of Amanita muscaria each from a different developmental stage. Results show that the maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroom stage of development. Moreover, it was also found that this concentration progressively decreases as the fungus ages. The developed method is a simple but effective method for the quantification of muscimol, a widely important metabolite for the pharmaceutical industry as a possible treatment for tardive dyskinesia and Parkinson’s disease.
Highlights
Commonly known as “fly agaric” or “fly amanita”, is one of the best known psychoactive mushrooms in the world due to its psychotropic properties [1]. is fungus is distinguished by a bright red cap featuring small white warty spots, and it is generally found in Europe, Africa, Asia, and the Americas
In Colombia, it was introduced as a symbiont with pine and eucalyptus trees [2]. e main psychoactive constituents of this basidiomycete are neurotoxins ibotenic acid (IBO) and muscimol (MUS), both of which are of interest due to their hallucinogenic and pharmacological properties [3]
One study quantified both analytes by derivatizing them with dansyl chloride (DNS-Cl) [9], while another study described a method based on the interaction of sensitive ions which allowed for the simultaneous detection of IBO and MUS [10]
Summary
Commonly known as “fly agaric” or “fly amanita”, is one of the best known psychoactive mushrooms in the world due to its psychotropic properties [1]. is fungus is distinguished by a bright red cap featuring small white warty spots, and it is generally found in Europe, Africa, Asia, and the Americas. Considering that most available methods for the quantification of muscimol require a chemical transformation of the analyte, the following study proposes a simple derivatization-free, RP-HPLC-based method that allows for the quantification of muscimol (MUS) in aqueous extracts of different developmental stages of A. muscaria. This method provides a way of identifying which developmental stage of A. muscaria will yield the highest concentration of MUS in order for it to be used as a GABAA receptor agonist
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