Abstract
Canine adenovirus type 2 (CAdV-2) is often found in co-infections with other pathogens causing canine infectious respiratory disease (CIRD). Rapid, efficient, and convenient pathogen detection is the best approach for early confirmatory diagnosis. In this study, we developed and evaluated a rapid real-time recombinase polymerase amplification (RPA) assay for detection of canine adenovirus 2 (CAV), which can detect CAV within 15 min at 39°C. The detection limit that assay was 214 copies/μl DNA molecules per reaction. The specificity was indicated by a lack of cross-reaction with canine distemper virus (CDV), canine coronavirus (CCV), and canine parvovirus (CPV). Field and clinical applicability of this assay were evaluated using 86 field samples. The coincidence rate of the detection results for clinical samples between CAV-RPA and qPCR was 97.7%. In summary, the real-time CAV-RPA analysis provides an efficient, rapid and sensitive detection method for CAV.
Highlights
Canine adenovirus (CAV) is a member of the genus Mastadenovirus, family Adenoviridae [1]
Canine adenovirus type-2 was first recovered in 1961 from dogs with laryngotracheitis [4], it is associated with mild infection of the respiratory tract and causes commonly widespread infectious tracheobronchitis (ITB) [5]
We developed a rapid and sensitive recombinase polymerase amplification (RPA) assay for detection of CAV-2, based on the E3 gene
Summary
Canine adenovirus (CAV) is a member of the genus Mastadenovirus, family Adenoviridae [1]. Canine adenovirus-type 1 strains have been reported worldwide from carnivore species included in the Canidae, Ursidae, and Mustelidae families [3]. Canine adenovirus type-2 was first recovered in 1961 from dogs with laryngotracheitis [4], it is associated with mild infection of the respiratory tract and causes commonly widespread ITB [5]. It has been detected in the brains of dogs with neurological signs [6]. With CAV-2, the route of infection is oronasal; it replicates efficiently in the respiratory
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