Abstract
The article presents results of the studies on development of a rapid scheme for isolation and bacteriological identification of Aeromonas veronii bacteria, which induce aeromonosis of salmon and cyprinids, as well as A. salmonicida subsp. salmonicida, A. hydrophila, A. sobria, A. eucrenophila A. сaviae. The experiments were carried out on the bacterial strain Aeromonas veronii biogroup sobria - АТSS 9071. The scheme consists of three stages: the first is the isolation of microorganisms from the test sample using the A.v.1-UlGAU accumulative nutrient medium of the original author's formula; the second stage is reseeding of colonies typical for Aeromonas veronii on the author's dense differential diagnostic medium A.v.2-UlGAU; the third stage is the study of physiological and biochemical parameters: mobility specification, oxidase production, xylose utilization, pigment formation of grown bacterial colonies on GRM agar with tyrosine, decarboxylation of lysine and ornithine, arginine hydrolysis, lactate fermentation. The proposed isolation and identification method will allow to differentiate Aeromonas veronii from associated bacteria, including A. salmonicida, A. hydrophila, A. caviae, within 120 hours, at relatively low costs for nutrient media and reagents.
Published Version
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