Abstract
In Mauritius, substantial decrease in potato yields due to late blight are reported annually and it has become necessary to track the genetic identity of local Phytophthora infestans strains. The Random Amplified Polymorphic DNA (RAPD) technique is a low-cost and simple genetic characterisation tool that has been widely used for molecular fingerprinting but which requires extensive optimisation. The aim of this study was to carry out a series of experiments to optimise a RAPD protocol for routine P. infestans typing. Amplifications performed with DNA template concentration of 30-50 ng/μl, primer concentration of 2.0 − 3.0 μM and MgCl2 concentration of 3.0 mM gave the best RAPD profiles. These optimised conditions were used to carry out RAPD fingerprinting of 7 P. infestans isolates and codified data were used to construct a consensus dendogram, which grouped the 7 isolates into 3 distinct clusters, consistent with results obtained from other molecular characterisation techniques.
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