Abstract
Human pluripotent stem cell (hPSC)-derived organoids and cells have similar characteristics to human organs and tissues. Thus, in vitro human organoids and cells serve as a superior alternative to conventional cell lines and animal models in drug development and regenerative medicine. For a simple and reproducible analysis of the quality of organoids and cells to compensate for the shortcomings of existing experimental validation studies, a quantitative evaluation method should be developed. Here, using the GTEx database, we construct a quantitative calculation system to assess similarity to the human organs. To evaluate our system, we generate hPSC-derived organoids and cells, and detected organ similarity. To facilitate the access of our system by researchers, we develop a web-based user interface presenting similarity to the appropriate organs as percentages. Thus, this program could provide valuable information for the generation of high-quality organoids and cells and a strategy to guide proper lineage-oriented differentiation.
Highlights
Human pluripotent stem cell-derived organoids and cells have similar characteristics to human organs and tissues
Evaluating differentiation status using key tissue-specific markers can be an efficient strategy for the design and optimization of differentiation methods, but evaluating the similarity between human tissue and differentiated cells/ organoids is difficult because experimental validation is laborious and time consuming
Development of a quantitative calculation system to assess the similarity of Human pluripotent stem cell (hPSC)-derived organoids and cells to organs
Summary
Human pluripotent stem cell (hPSC)-derived organoids and cells have similar characteristics to human organs and tissues. To facilitate the access of our system by researchers, we develop a web-based user interface presenting similarity to the appropriate organs as percentages This program could provide valuable information for the generation of high-quality organoids and cells and a strategy to guide proper lineage-oriented differentiation. Advances in threedimensional (3D) culture systems have enabled the development of complex organotypic models using 3D organoids that differentiate from stem cells into tissue-like miniature analogs that recapitulate complex tissue-specific cell compositions, architectures, and functions[11,12,13] These 3D organoid technologies provide an opportunity to study human development and disease in depth by assessing cellular interactions, location, and structural changes[14,15]. We need to build a web-based analytics platform that is readily available to researchers
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