Abstract
An attractive electrochemical sensor of poly(3,4-ethylenedioxythiophene)/reduced graphene oxide electrode (PrGO) was developed for an electrochemical technique for uric acid (UA) detection in the presence of ascorbic acid (AA). PrGO composite film showed an improved electrocatalytic activity towards UA oxidation in pH 6.0 (0.1 M PBS). The PrGO composite exhibited a high current signal and low charge transfer resistance (Rct) compared to a reduced graphene oxide (rGO) electrode or a bare glassy carbon electrode (GCE). The limit of detection and sensitivity of PrGO for the detection of UA are 0.19 μM (S/N = 3) and 0.01 μA/μM, respectively, in the range of 1–300 μM of UA.
Highlights
Uric acid (UA) is present in human biofluids like urine and blood. It is a protein metabolism byproduct which is found in large amounts in certain foods that may cause some harm because humans do not have any enzyme to break down uric acid, UA will accumulate and contribute to diseases like gout or kidney stones
Poly(3,4-ethylenedioxythiophene) (PEDOT), polyaniline (PANI) and polypyrrole (Ppy) are examples of conducting polymers (CPs) that have been reported as useful materials for making biosensors [3,4] because it shows a high selectivity for analytes in the oxidized state of CP [5]
The results indicated that PrGO composite was well-suited and well-defined for the fabrication of a UA biosensor in the presence of ascorbic acid (AA) due to its excellent electrochemical performance
Summary
Uric acid (UA) is present in human biofluids like urine and blood. It is a protein metabolism byproduct which is found in large amounts in certain foods that may cause some harm because humans do not have any enzyme to break down uric acid, UA will accumulate and contribute to diseases like gout or kidney stones. Poly(3,4-ethylenedioxythiophene) (PEDOT), polyaniline (PANI) and polypyrrole (Ppy) are examples of conducting polymers (CPs) that have been reported as useful materials for making biosensors [3,4] because it shows a high selectivity for analytes in the oxidized state of CP [5]. GO has limitations which lead to a low surface area and low conductivity [20] These problems can be overcome by reducing the GO, forming reduced graphene oxide (rGO) to obtain high electron transfer, high conductivity and good electrochemical activity [21] and when incorporated with PEDOT it produces an excellent biosensor platform for the detection of uric acid. PrGO revealed a low detection limit towards oxidation of UA and it possesses an excellent analytical performance, with good stability, high sensitivity and selectivity for UA. The PrGO composites were characterized using Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and field emission scanning electron microscopy (FESEM)
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