Abstract
In this study, a potentiometric ion-selective electrode (ISE) was developed in order to quantifyractopamine. Ractopamine is a phenylethanolamine with s2-adrenergic agonist properties; was in fact developed for treating respiratory diseases; and is used as tocolytic, bronchodilator and heart tonic in human and veterinary medicine (1). Ion-pair of Ractopamine-Tetraphenylborate was synthesized. The synthesized ion pair was used as ionophore in the structure of electrode membranes. For membrane optimization, PVC membrane ion-selective electrodes were produced in various compositions using the synthesized ion pairs and potentiometric performance characteristics of these electrodes were investigated. It was determined that the electrode with the composition of 5% Rac-TPB+32%PVC+62.6%DBF+0.4% KTpClPB (Potassium tetrakis (4-chlorophenyl borate) demonstrated the best potentiometric performance characteristics. The linear range, slope, limit of quantification, pH range, and response time of this electrode were determined to be 1.0×10 -5 -1.0×10 -1 M, 51.0 mV/decade, 1.0×10 -5 M, 3.0-5.0, and< 5s, respectively. The electrode has demonstrated a highlyrepeatable potentiometric response. It was applied in pigswill using ractopamine selective electrode .
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