Abstract
Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high sensitivity and specificity in phosphate-buffered saline buffer, with a half maximal inhibition concentration value of 1.77 ng mL−1 and a limit of detection of 0.09 ng mL−1. The average recovery in spiked chicken muscle samples was 84.4–114.8%, with a relative standard deviation below 8.1%. To validate the developed icELISA method, samples spiked with different levels of TCS were analyzed by icELISA and ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The consistency of results detected by icELISA and UPLC–MS/MS demonstrated that immunoaffinity cleanup followed by icELISA could also be used for other food matrices.
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