Abstract
Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitive and quantitative detection of diseases can be performed using fluorescent immunochemical assay with specific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitative biosensor to transmit diagnostic results to a control center in order to systematically prevent disease outbreaks. In this study, we developed a novel fluorophore, coumarin-derived dendrimer, with high emission intensity, strong signal brightness, and high photostability. It is easily coupled with biomolecules and emits strong and stable fluorescence at 590 nm with excitation at 455 nm. Application to fluorescent immunochromatographic test (FICT) showed that the novel coumarin-derived dendrimer bioconjugate could detect antigens at amount as low as 0.1 ng. The clinical results and the spectral characteristics of the novel coumarin-derived dendrimer open, for the first time, the possibility of developing a cost/energy efficient LED-based portable quantitative biosensor for point-of-care (POC) disease diagnosis, which can permit real time monitoring (U-healthcare system) by a disease control center.
Highlights
Accurate and prompt diagnosis is one of the keys for effective disease management
A novel fluorophore derived from coumarin with high fluorescence emission intensity, strong signal brightness, and high photostability has been developed
Coumarin was chosen because it is relatively easy to synthesize and thereby easy to modify to make a variety of derivatives
Summary
Accurate and prompt diagnosis is one of the keys for effective disease management. Rapid diagnosis is especially important for infectious diseases such as swine flu and avian flu to prevent their spread. The result demonstrated that FLISA system using the novel coumarin-derived dendrimer is very specific once bioconjugating monoclonal antibody is specific for antigen (Figure S1A and Table S1) These suggest that the novel coumarin-derived dendrimer is highly sensitive and applicable for immunochemical assay for disease diagnosis. FICT using clinical samples of HCV and HIV infection was further performed to test the possibility of false positive detection in the FICT system and it was found that the FICT system was highly specific (Figure S1B) These suggest that the novel coumarin-derived dendrimer paired with specific antibodies is extremely sensitive, specific and applicable for the development of a rapid and quantitative biosensor using FICT as a diagnostic method
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