Abstract
A straightforward, delicate, and fast elite thin-layer chromatographic system (high-performance thin-layer chromatography [HPTLC]) has been produced and validated for the quantitative determination of berberine in Tinospora cordifolia. The chromatographic development was completed on HPTLC plates precoated with silica gel 60 F254 utilizing a blend of methanol, acetic acid, and water (8:1:1, v/v) as the mobile phase. Detection was completed densitometrically at 366 nm. The RF estimation of berberine was observed to be 0.71 ± 0.02. The system was validated according to the International Conference on Harmonization (ICH) guidelines regarding linearity, precision, accuracy, robustness, and so forth. The calibration curve was observed to be straight over a scope of 120–360 ng spot−1 with a regression coefficient of 0.971. The accuracy was observed to be as high as 98.56%, and the relative standard deviation (% RSD) values for intra-day and between-day variations were under 2%. The system showed high affectabil...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: JPC - Journal of Planar Chromatography - Modern TLC
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
