Abstract
Due to the rise in substitution and adulteration of herbal products, as well as the lack of genetic information on Hibiscus plants, more molecular markers are needed to understand the genetic diversity and avoid their misidentification. There are many allelic variants of the functional genes in Hibiscus and other plants, which control their respective phenotypes and other characteristics. Identifying alleles of the desired trait by determining diversification through gene-typing allele-specific markers for authentication is, therefore, a potent strategy. The purpose of this study was to use insertion/deletion (InDel) markers to identify Hibiscus syriacus cultivars. We developed a novel InDel marker for Korean Hibiscus ‘Simbaek’, based on the trnL-F region of the chloroplast gene. Through this InDel site, a modified specific primer pair and a novel multiplex polymerase chain reaction (PCR) system were developed for specific identification of the Korean Hibiscus Simbaek cultivar. The molecular markers developed in this study were highly specific and accurately authenticated as Simbaek from the five main cultivars of H. syriacus. Taken together, the described method is a potential tool for the identification and selection of germplasm resource of Simbaek cultivar.Graphical
Highlights
Hibiscus syriacus is a deciduous shrub belonging to the genus Malvaceae
In addition to its ornamental value, dried flowers and root bark of H. syriacus are known for their anticancer [2], antidepressant [3], and antioxidant [4] properties
American Hibiscus (Hibiscus sabdariffa) is often regarded as an adulterant of Korean Hibiscus which brings a lot of trouble to consumers.Traditionally, confirming the authenticity of species or cultivars relies on morphological characteristics, such as growth environment, floral morphology, and other characteristics of the plant [5]
Summary
Hibiscus syriacus originated in the Korean Peninsula and southern China, and later spread to the Indian subcontinent and middle-eastern regions. H. syriacus is a common ornamental species with white, pink, red, or purple flowers [1]. In addition to its ornamental value, dried flowers and root bark of H. syriacus are known for their anticancer [2], antidepressant [3], and antioxidant [4] properties. American Hibiscus (Hibiscus sabdariffa) is often regarded as an adulterant of Korean Hibiscus which brings a lot of trouble to consumers.Traditionally, confirming the authenticity of species or cultivars relies on morphological characteristics, such as growth environment, floral morphology, and other characteristics of the plant [5]. As majority of the herbal products are packaged in the form of powders that do not bear the original features of the plants, identification of the latter by morphological and histological techniques becomes difficult
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