Development of a highly sensitive ELISA and immunochromatographic strip to detect pentachlorophenol

Abstract

ABSTRACTA sensitive monoclonal antibody for targeting pentachlorophenol was prepared with an immunization procedure and cell fusion. The antibody was characterized with an indirect competitive enzyme-linked immunosorbent assay (ELISA), which had an IC50 of 0.2 ng/ml for pentachlorophenol under optimized conditions. The cross-reactivity of the ELISA to related compounds was very low. The recovery of pentachlorophenol was 99.2–105% in water samples and 89–110.9% in fish samples. A simple, rapid, and sensitive immunochromatographic strip was produced based on the monoclonal antibody, with a qualitative visual limit of detection of 2 ng/ml in water samples and 10 ng/ml in fish samples, indicating its potential utility in monitoring pentachlorophenol in the field.