Abstract
Tomato yellow leaf curl virus (TYLCV) causes serious yield reductions in China. The use of certain resistance genes in tomato varieties has alleviated the impact of the virus to a certain extent. Recently, varieties with the Ty-1, Ty-2, or Ty-3 genes lost their resistance to TYLCV in some areas in China. New genes should be introduced into tomato to maintain the resistance to TYLCV. Tomato line AVTO1227 has excellent resistance to disease due to the resistance gene ty-5. In this study, we screened different types of markers in a tomato F2 population to compare their accuracy and efficiency. The sequencing analysis results were consistent with the high resolution melting (HRM) marker genotype and field identification results. The result confirmed that the functional marker of ty-5 was accurate and reliable. The single nucleotide polymorphism-based HRM genotyping method established in this study can be used for the selection of breeding parent material, gene correlation analysis, and molecular marker-assisted breeding.
Highlights
Academic Editors: Giovanni BubiciTomato (Solanum lycopersicum) belongs to the Solanaceae family, originating from SouthAmerica
Susceptible tomato lines infected by Tomato yellow leaf curl virus (TYLCV) typically exhibit severe curling and yellowing of the top leaves, in addition to slow and stunted growth [2]
Breeding TYLCVresistant tomato cultivars offers an attractive method for controlling this disease and could fundamentally resolve the devastation caused by TYLCV [5,6,7,8]
Summary
Tomato (Solanum lycopersicum) belongs to the Solanaceae family, originating from South. Ty-2 was introgressed into tomato via Solanum habrochaites strain B6013 and was found to be located on chromosome 11. This gene encodes a nucleotide-binding leucine-rich repeat protein [16,17]. Ty-6 is a novel resistance gene that is located on chromosome 10 and is derived from Solanum chilense strain LA2779 [18]. The recessive gene ty-5 was mapped on chromosome 4 and identified from breeding line TY172 derived from Solanum peruvianum [19,20]. The objectives of this study included to establish a MAS breeding platform, enrich the collection of resistant tomato varieties, speed up breeding selection, and provide a basis for clarifying the resistance mechanism of ty-5 resistance-related genes
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