Abstract
Abstract We have developed a high-throughput, full field-of-view (FOV) spectroscopic single-molecule fluorescence (sSMF) instrument, overcoming distortion issues with gratings and prisms. Our calibration techniques ensure spectral uniformity across the whole FOV, and our two-step Gaussian fit method enables better than 2 nm precision in spectral peak determination for accurate molecule identification, making it a valuable tool for multi-color single-molecule detection and analysis.
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