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Abstract
<p><strong>Objective: </strong>A simple and sensitive high-performance thin–layer chromatographic (HPTLC) method was developed for the quantification of ursolic acid in the leaves of three species of genus <em>Tecoma</em> (<em>Tecoma gaudichaudi</em> DC, <em>Tecoma capensis</em> (Thunb.) Lindl, <em>Tecoma stans</em> (L.) Juss. ex Kunth) and genus <em>Tabebuia</em> (<em>Tabebuia rosea</em> Bertol) belong to family Bignoniaceae.</p><p><strong>Methods: </strong>Chromatography was performed on Silica gel 60 F254 precoated HPTLC Plates with optimised mobile phase pet ether: ethyl acetate: formic acid (7:3:0.5, v/v/v). The plate was derivatized with p-anisaldehyde reagent and scanned at 540 nm. The developed method was found to give a compact spot for ursolic acid at R<sub>f </sub>value 0.43±0.01. The method was validated using International Council for Harmonization (ICH) guidelines, including linearity, precision, accuracy, and robustness.</p><p><strong>Results: </strong>Ursolic acid was found to be present in four species, i.e., <em>Tecoma gaudichaudi</em> DC (1.48%w/w), <em>Tecoma capensis</em> (Thunb.) Lindl. (0.79%w/w), <em>Tecoma stans</em> (L.) Juss. Ex Kunth (1.11%w/w), <em>Tabebuia rosea</em> (Bertol.) (1.13%w/w). A good linearity relationship was found to be (200-1400ng band−1) with a correlation coefficient (r<sup>2</sup>) value of 0.9946 with ursolic acid. Limit of detection and limit of quantification was considered to be 40.66, 123.21 ng per band respectively. The developed method was found to be accurate and precise with 1.32%, 1.19% (%RSD) for interday and intraday precision. The accuracy of the method was performed by recovery studies at three different concentration levels, and the average percentage recovery was found to be 98.05% for ursolic acid.</p><p><strong>Conclusion</strong>:<strong> </strong>The proposed method for the quantitation of ursolic acid was found to be reproducible and simple.</p>
Highlights
Bignonia Linn (Bignoniaceae) is a monotypic genus of woody climbers, native to North America and mostly grown for ornament in the tropics of the old world [1]
The developed mobile phase was found to be effective in the separation of components in samples Tecoma gaudichaudi DC (Sample 1), Tecoma capensis (Thunb.) Lindl. (Sample 2), Tecoma stans (L.) Juss
Pre-analyzed samples were spiked with 50%, 100% and 150% of the standard ursolic acid and analysed by the proposed high-performance thin–layer chromatographic (HPTLC) method
Summary
Bignonia Linn (Bignoniaceae) is a monotypic genus of woody climbers, native to North America and mostly grown for ornament in the tropics of the old world [1]. Bignoniaceae family was having 100 genera and more than 750 plant species observed in various tropical regions of India. Known numbers of this family are Bignonia, Tecoma, Catalpa, Tabebuia and Jacaranda. These are succulent herbs, shrubs, stem sometimes reduced to a rhizome or tuber. Numerous species of this family are observed as poisonous to leeches [2]. In Charak, Sushruta, the root, bark, stem and leaf of some species of Bignoniaceae family is useful for snake bite, the stem and wood for scorpion sting. In Bangladesh whole plant of Tecoma gaudichaudi DC use of a remedy for diabetes and infertility problems [3]
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