Development of a Cuvette-Based LSPR Sensor Chip Using a Plasmonically Active Transparent Strip.

Seo Yeong Oh,Youngjin Cho,Sung-Chan Jang,Nam Su Heo,Yun Suk Huh,Vivek K Bajpai,Gyeongsik Ok

doi:10.3389/fbioe.2019.00299

Abstract

This research demonstrates the development of a transmission-mode localized surface plasmon resonance (LSPR) sensor chip using a cuvette cell system for the sensitive detection of a biomolecule marker such as C-reactive protein (CRP). In order to develop a highly sensitive LSPR sensor chip, plasmonically active gold nanoparticles (AuNPs) were decorated onto various transparent substrates in the form of a uniform, high-density single layer using a self-assembly process. The transparent substrate surface was modified with amine functional groups via (3-Aminopropyl)triethoxysilane (APTES) treatment, and the ligand concentration and temperature (0.5% APTES at 60°C) were then optimized to control the binding energy with AuNPs. The optimized plasmonically active strip was subsequently prepared by dipping the amine-functionalized substrate into AuNPs for 8 h. The optimized plasmonic strip functionalized with anti-CRP was transformed into a portable LSPR sensor chip by placing it inside a cuvette cell system, and its detection performance was evaluated using CRP as a model sample. The detection limit for CRP using our LSPR sensor chip was 0.01 μg/mL, and the detection dynamic range was 0.01–10 μg/mL with a %CV of <10%, thus confirming its selectivity and good reproducibility. These findings illustrate that the highly sensitive portable LSPR biosensor developed in this study is expected to be widely used in a diverse range of fields such as diagnosis, medical care, environmental monitoring, and food quality control.

Highlights

A biosensor utilizes a biologically recognized functionalized substrate to detect a target molecule and a detector or reader capable of converting biochemical interactions into physical and/or chemical signals (Sepúlveda et al, 2009)
Plasmon Absorption Characteristics of Various Transparent Substrates In this study, we developed a portable localized surface plasmon resonance (LSPR) sensor chip with strong potential as a field detection device by optimizing the binding characteristics between plasmonic a method of uniformly depositing gold nanoparticles (AuNPs) and a transparent substrate
In a transmission-mode LSPR sensor chip, the plasmonic substrate on which AuNPs are deposited efficiently produces resonance phenomena depending on the incident light and the physical reaction conditions, such as the material type and thickness of the substrate, generating changes in the plasmon absorption spectrum that can act as a detection signal

Summary

Introduction

A biosensor utilizes a biologically recognized functionalized substrate to detect a target molecule and a detector or reader capable of converting biochemical interactions into physical and/or chemical signals (Sepúlveda et al, 2009). Other studies have reported the development of sensitive LSPR sensors that utilize different receptor types, including aptamers, antibodies, peptides, and chemicals, in order to optimize the selective binding force with the target molecule (Zhang et al, 2014; Li et al, 2016; Thakur et al, 2017; Lee et al, 2018) Despite this past research on plasmonic nanoparticle synthesis and specific receptor screening (Sefah et al, 2010; Wu et al, 2016b; Ozgul et al, 2019), there have been few reports on the optimal methodology for the deposition of plasmonic nanoparticles (Galush et al, 2009; Hsu et al, 2011; Coskun et al, 2014; Im et al, 2014) and the fabrication of portable substrates that can provide stable and reproducible plasmonic signals

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Conclusion