Development of a bioreactor for high density culture of hairy roots

Abstract

The effects of agitation and aeration on the growth of carrot hairy roots were investigated. When hydrodynamic stress index was above 0.001 cm/s, the growth rate of hairy roots decreased sharply. When volumetric O2 transfer coefficient was high, the specific growth rate was also high. However, the specific growth rate approached the maximum value when the volumetric O2 transfer coefficient was over 4 h−1 . It is therefore necessary to maintain low hydrodynamic stress and high volumetric oxygen transfer for high density culture of hairy roots. By considering hydrodynamic stress and oxygen transfer, a novel bioreactor type was suggested for hairy roots cultivation.