Abstract
The Ah-Immunoassay method was developed using a Bio-MEMS approach and applied to the evaluation of dioxin toxicity in this study. The aim of the research is to develop a real time sensing system for the analysis of Dioxins, generated at the air flue by using a novel micro instrument combining the biological field with micro instrumentation. The micro instrument was fabricated from Si and glass, employing photolithographic techniques. A prototype model of the system was established. Micro-beads, coated with Avidine, were used as a reaction medium. The reaction chamber was first filled with the micro-beads and then the receptor immunoassay was carried out in the micro chamber. Poly-aromatic hydrocarbon, having a highly flat structure, was used as a test sample. The reactivity was evaluated using fluorometry, the fluorescence being detected by fluorescent microscopy.
Published Version
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