Abstract

Cloning of small DNA segments has been established using Escherichia coli plasmids. The cloned DNA can be transferred to various cells using transformation. In contrast, cloning of large DNA segments of more than several hundred kilobase pairs has been limited to the Bacillus subtilis genome cloning system. The advantage of giant DNA cloned by B. subtilis is that all kinds of gene editing can be implemented by the high and strict natural transformation ability of the host. However, the following transfer step of giant synthesized and edited genomes to different cell systems require a special system by avoiding exposure in liquid. The use of a conjugational plasmid pLS20 that was developed for 20 years improves the B. subtilis genome vector establishment process from scratch. The use of the unique B. subtilis genome vector system from synthesis to transmit genomes is now being manipulated and summarized for the first time.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.