Abstract
This paper describes the experimental realization and characterization of a versatile single particle detection apparatus. The system utilizes a novel particle beam inlet that can serve as either an on-line particle concentrator (i.e., all diameters confined in a narrow beam) or as a segregator (i.e., selected diameters confined in a narrow beam) and can be operated in a high-speed mode as well as in a low-speed mode, thus allowing different interaction times between the particles and the laser beam. An aerodynamic sizing technique has been incorporated into the system to provide rapid, real-time, and high-resolution sizing. Parameters such as transmission efficiency and size-segregation efficiency have been measured. The performance of the instrument has been demonstrated by on-line detection of spectrally resolved and time resolved fluorescence detection from airborne dye-doped particles and aerosolized endogenous fluorophores found in biological agents.
Highlights
Airborne particles, biological or nonbiological in origin, play a crucial role in many health and environmentally related issues [1]
In addition to several important studies regarding the generation of particle beams and their focusing properties, in the last 20 years there has been a great interest in the development of methods for realtime detection and identification of individual airborne particles, including bioaerosols and pathogens [11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47]
Given the fact that the ambient aerosols cover more than five orders of magnitude in size, an ideal particle beam inlet is one whose size focusing range can be adjusted over a broad range to accommodate different applications
Summary
Biological or nonbiological in origin, play a crucial role in many health and environmentally related issues [1]. Laser Chemistry to simultaneously transmit a wide range of particle sizes The representatives of this type of inlet are aerodynamic lenses. Given the fact that the ambient aerosols cover more than five orders of magnitude in size, an ideal particle beam inlet is one whose size focusing range can be adjusted over a broad range to accommodate different applications. The aim of this paper is to describe the experimental realization and characterization of a system that integrates an aerodynamic particle beam inlet with a size measurement and fluorescence analysis system. Our current research interests are to combine this apparatus with time resolved fluorescence and laser-induced plasma measurements to improve the discrimination of different types of biological particles on a real time basis [43, 51]. An overview of the system design and characterization is provided
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