Abstract
Introduction: The aim of this work is to establish a novel, simple, precise and sensitive UV-AUC spectrophotometry method for estimation of Pyrazinamide from bulk and in-Pharmaceutical formulation and validate it according ICH guidelines. Pyrazinamide is used for the treatment of tuberculosis.
Highlights
The aim of this work is to establish a novel, simple, precise and sensitive UV-area under curve (AUC) spectrophotometry method for estimation of Pyrazinamide from bulk and in-Pharmaceutical formulation and validate it according ICH guidelines
Pyrazinamide (PZA) is an amide of pyrazinoic acid (PC) used in the combination treatment of tuberculosis recommended by WHO
It was found that Pyrazinamide and its degradation product was analyzed by different HPLC methods either alone [4] or in combination with other drugs such as rifampicin, ethambutol hydrochloride and isoniazid [5,6]
Summary
The aim of this work is to establish a novel, simple, precise and sensitive UV-AUC spectrophotometry method for estimation of Pyrazinamide from bulk and in-Pharmaceutical formulation and validate it according ICH guidelines. A stock standard solution was prepared by dissolving 10 mg of Pyrazinamide in to 100 mL volumetric flask volume was made by methanol up to the mark, the obtaining concentration was 100μg/mL. After suitable dilutions, it scans in the UV-visible range 200 - 400 nm. Pyrazinamide (PZA) is an amide of pyrazinoic acid (PC) used in the combination treatment of tuberculosis recommended by WHO.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
