Abstract
In this study, two analytical methods viz. UV spectrophotometry and RP-HPLC were developed for the evaluation of Amalaki in the marketed herbal formulation. The amount of gallic acid estimated in the marketed formulation complies with the standard (not less than 1% w/w of gallic acid) specified in the official monograph of Amalaki. The developed methods showed good linearity, accuracy, precision, ruggedness, robustness, specificity, LOD and LOQ. Results of validation studies were found satisfactory with % RSD values of less than 2% indicating good specificity, validity and reliability of the developed methods. Both analytical methods are claimed to be simple, accurate and precise. Present methods can, therefore, be applied widely for the routine analysis of marketed formulations or any crude traditional preparations of Amalaki based on the quantitative determination of gallic acid at a reasonable cost with simple analytical set up.
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