Development and validation of Novel UV-Spectrophotometric method for estimation of Cyamemazine Tartarate for simple, novel, rapid and cost effective analysis

Abstract

Simple, novel, rapid and cost effective UV-spectrop hotometric method has been developed and validated for estimation of CYMT in bulk and tablet formulation. CYMT is a Phenothiazine derivative fr om the class of typical antipsychotic and a new drug for t he treatment of Schizophrenia. As no analytical met hod was seen in literature for determination of CYMT; we re port here, development and validation of simple, no vel UVspectrophotometric method for analysis of CYMT. Drug was found to be soluble in water and was stable f or more than 72 h in same solvent when tested for benc h top stability; being ideal solvent, spectrophotom etric studies were carried out using distilled water and detection as well as quantitation was performed at wavelength maximum of 267.21 nm. Linearity curve for developed method was generated by measuring absorbance at specified wavelength maximum and plotting it against concentration. The method followed linearity in r ange of 2 - 12 µg/mL with a correlation coefficient of 0.999. The m ean recovery of 98.97 reflects accuracy for developed method and the method precision was found to be well within acceptable limits. The developed method was tested and validated for various paramet ers as per USP requirements and recent ICH guidelines (addendum 2005). The present UV-spectrophotometric estimation for CYMT was proved to be statistically accurate, precise, and sensitive. The applicability of method can be extended towards rapid routine determination of drug in bulk and pharmaceutical fo rmulations and it has the unique importance of bein g first and simple UV-Spectrophotometric analytical method for the determination of CYMT in bulk and in pharmaceutical formulation