Development and validation of ultraviolet spectrophotometric method for Saquinavir estimation in bulk and dosage form

Abstract

We developed and validated a simple, rapid, sensitive, precise, and reproducible specific UV spectrophotometric technique for determining Saquinavir in bulk medication and pharmaceutical dosage form. Methods: As per ICH recommendations, a simple double-beam UV spectrophotometric technique has been designed and validated with several criteria, such as linearity, precision, repeatability, the limit of detection (LOD), limit of quantification (LOQ), and accuracy. Results: UV-visible spectrophotometric approach, measurement of absorption at the maximum wavelength in 10 mL methanol, and volume made with water solvent system as reference Saquinavir was discovered at 239 nm. The medicine followed Beer's rule and showed a good correlation. Beer's law was followed for Saquinavir in the 1-5µg/ml concentration range, with a correlation coefficient of 0.999. Saquinavir's LOD and LOQ were determined to be 0.56 μg/ml and 2.78 μg/ml, respectively. The proposed method is simple, precise, accurate, and reproducible, and it may be utilized for routine analysis of Saquinavir in bulk and tablet dosage form.