Abstract
In the present work, RP-HPLC method was developed for the analysis of Moxifloxacin in the marketed ophthalmic formulation. This eye solution is used to treat infections of the eye including bacterial conjunctivitis. The chromatographic separation was achieved with UV detection at 292 nm. The stationary phase used is Sunfire C18 column (250 mm × 4.6 mm, 5μm particle size column). The mobile phase contains a mixture of methanol: ACN: water with 0.1% TEA in isocratic elution mode. The total run time was 10min. The proposed method is found having linearity in the concentration range of 2-12μg/ml with co-relation coefficient of 0.999. This method was validated in accordance with International Conference on Harmonization (ICH) guidelines. The developed method is found simple and accurate. Due to its simplicity, rapidness, precision and accuracy, it may be used for laboratory analysis.
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