Abstract
This study was conducted to identify the biological active substances (BAS) from the fruit of Tetrapleura tetraptera obtained from Ghana. TLC, UV Spectroscopy and differential spectrophotometry methods of analysis were employed in this study. Identified BAS included flavonoids, polysaccharides and amino acids. Following state pharmacopoeia of Ukraine (SPU) requirements to validate the developed techniques were employed in this study, and the following validation properties specificity, linearity, range, precision accuracy (convergence) and robustness was determined. These characteristics did not exceed the eligibility criteria as stated in (SPU) Appendix 2. Thus the technique can be made to the SPU monograph on fruits of Tetrapleura tetraptera.
Highlights
The medicinal plant Tetrapleura tetraptera belongs to the family Mimosaceae
This study was conducted to identify the biological active substances (BAS) from the fruit of Tetrapleura tetraptera obtained from Ghana
Following state pharmacopoeia of Ukraine (SPU) requirements to validate the developed techniques were employed in this study, and the following validation properties specificity, linearity, range, precision accuracy and robustness was determined
Summary
The medicinal plant Tetrapleura tetraptera belongs to the family Mimosaceae. It is known as prekese by the Akan population in Ghana. Tetrapleura tetraptera is a perennial plant with dark green leaves and mostly found in the rain forest belt of the West, Eastern and Central sub-regions on the African continent [2] [3]. The major bioactive compounds found in Tetrapleura tetraptera are saponins, tannins, phenols, flavonoids and glycosides [3]. Logoyda the indigenous population in Africa for the treatment of hypertension, asthma, schistosomiasis and epilepsy [4]. The present study examines the development of methods for identification and quantification of biologically active substances (BAS) found in the fruit of Tetrapleura tetraptera
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