Abstract
Four analytical methods have been developed and validated for the quantitation of β-blockers (propranolol, metoprolol, atenolol and bisoprolol) using high-performance liquid chromatography (HPLC) with UV detection. Excellent proportionality, precision and accuracy were achieved by the assays. The use of only one kind of reverse-phase column and a mobile phase for all β-blockers permitted the analysis of a large number of samples in a short time. Their stability in solution was also studied. The results show that the β-blockers assayed are stable for at least 84 h at 90°C.
Published Version
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