Abstract
Solanesol is the starting material for many high value biochemicals, including Co-enzyme Q10 and vitamin-K analogues. The aim of the current study was to develop and validate a reliable and fast analytical procedure for the determination of solanesol in Nicotianatabacum using high-performance thin layer chromatography (HPTLC) method. The method was developed on TLC aluminium plates precoated with silica gel 60F-254 using solvent system hexane: ethyl acetate (5:1, v/v), which gives compact spot of solanesol (Rf value 0.41 ± 0.02). Densitometric analysis of solanesol was carried out in the absorbance mode at 210 nm. The linear regression analysis data for the calibration plot showed good linear relationship with r = 0.9978 with respect to peak area, in the concentration rang 100-5000 ng per spot of solanesol. The limit of detection and quantification were 13 and 30 ng per spot, respectively. The proposed method was applied for quantitative estimation of solanesol in different parts of Nicotianatabacum from different geographical regions in India, which showed that maximum amount of solanesol was found to be present in leaf sample collected from Karnataka i.e. 3.52 mg/g. Statistical analysis proved that the method is repeatable, selective and accurate for the estimation of solanesol in Nicotianatabacum.
Highlights
Solanesol is a trisesquiterpenoid alcohol (Fig. 1) which was first isolated from tobacco [1]
Tobacco belongs to family Solanaceae and the plant is considered to be a good source of a large number of bioactive substances and is the starting material for many high value bio-chemicals, including Co-enzyme Q10 and vitamin-K analogues
An efficient procedure using high-performance thin layer chromatography (HPTLC) has been developed for rapid analysis and quantification of solanesol in different parts of N. tabacum from different geographical regions in India and successfully validated
Summary
Solanesol is a trisesquiterpenoid alcohol (Fig. 1) which was first isolated from tobacco [1]. Solanesol is a potentiating agent in many medicines. Solanesol can counteract the bacteria, diminish inflammation and hemostasis. It has strong activity of counteracting the cancer. With solanesol as its primary material, Co-enzyme Q is useful in the treatment of heart diseases, cancers, ulcers, wound and so on. A few methods for determination of solanesol in tobacco by HPLC [12,13], by GC [14], by HPLC-APCI-MS [15], and by LC-TMS [16], etc. To best of our knowledge no HPTLC method for quantitative estimation of solanesol in different parts of Nicotiana tabacum from different geographical regions has been reported. A simple, precise and accurate HPTLC method for rapid quantification of solanesol was developed and the method was validated to achieve the satisfactory precision and recovery
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