Abstract
A sensitive, selective, precise and stability-indicating high-performance thin-layer chromatography (HPTLC) method was developed and validated for analysis of carteolol both bulk drug and in formulation. The HPTLC method was chosen in order to generate better resolution and evade the tedious and prolonged sample preparation methods necessarily performed with HPLC methods when analyzing in samples. Carteolol was separated from the formulation on a silica gel 60F254 as the stationary phase. Elution was performed with mobile phase consisted of chloroform and methanol (5:1 v/v). Quantification of carteolol was carried out as per beak area observed from the densitometry at 254 nm absorbance mode. This system was found to give compact spots for carteolol (RF value of 0.31 ± 0.015, for three replicates). The method was validated for linearity, precision, robustness, limit of detection (LOD), limit of quantification (LOQ), specificity accuracy and degradation. Linearity was found to be in the range of 200–1200 ng...
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