Abstract
Lycopene is the red pigment richly found in many red -colored fruits and vegetables such as tomatoes, papaya, pink grapefruit, pink guava, and watermelon. It is an antioxidant that has various beneficent effects and protects against oxidative damage to lipids, protein, and DNA. It is a potent quencher of oxygen and most effective against cancer, cardiovascular diseases, osteoporosis, hypertension, male infertility, and neurodegenerative disease. Lycopene is synthesized by plants and microorganisms but not animals and humans. The main objective of this study is to develop and validate an HPLC assay method for the determination of lycopene in tomato, watermelon, and human blood. A simple assay method is an economical assay method, suitable for the determination of lycopene from plant and biological sources. A new accurate, specific, precise, high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of lycopene in tomatoes, watermelon and blood sample according to the International Conference on Harmonization (ICH) guidelines. Acetonitrile, Methanol, Water (65%, 20%, and 15%) was used as the mobile phase at a flow rate of 0.7ml/min. using a symmetry C18 column. The effluent was spectrophotometrically monitored at 484nm. The calibration curve was linear with a correlation coefficient of 0.9994. The average recovery was found to be 102.0%. The proposed HPLC method was successfully applied to quantify the amount of lycopene in different matrices.
Highlights
The study of antioxidants shows that vitamin selenium and lycopene all are very useful to reduce the risk of prostate cancer
It was shown in some studies that the lycopene level in serum and tissue was to be inversely related to the risk of prostate cancer and breast cancer
The observations which are achieved from epidemiological studies give mostly evidence to support the role of lycopene in the stoppage of cardiovascular disease
Summary
The name of lycopene is derived from the neo-Latin Lycopersicum, the tomato's species classification, Solanum Lycopersicum. Lycopene is the most powerful carotenoid quencher of singlet oxygen. Lycopene is one of the most efficient antioxidant’s carotenoids. The body is protected from the negative effects of oxidants by using natural pigment lycopene. The study of antioxidants shows that vitamin selenium and lycopene all are very useful to reduce the risk of prostate cancer. As a major carotenoid in human blood, lycopene protects against oxidative damage to lipids, proteins, and DNA. Lycopene is a potent quencher of singlet oxygen (a reactive form of Science Journal of Chemistry 2020; 5(2): 26-33 oxygen), which suggests that it may have comparatively stronger antioxidant properties than other major plasma carotenoids. Lycopene is a non-provitamin A carotenoid present in human blood and tissues. The major dietary sources of lycopene for the human are tomatoes and watermelons. Various cis-isomers constitute more than 60% of the total lycopene concentration
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