Abstract
The aim of this study was to develop a simple spectrophotometric method for the determination of Kanamycin (KM) in pure bulk form and in its pharmaceutical formulations. Being an amino group containing molecule, KM reacted with ascorbic acid to form a water soluble, purple-pink, 1:1 complex that showed two wavelengths maxima (λmax) at 390 nm and 530 nm. The color was developed after heating for 40 minutes at 100˚ C and remained stable for at least 48 hours. The validity of developed method was tested by analyzing KM under the optimum experimental conditions. Beers law was found valid over the concentration range (40-200μg /ml) with an excellent correlation coefficient (less than 0.999). The repeatability and reproducibility results showed a low relative standard deviation values (RSD % < 2), which reflected the precision of the developed method. The good percentages added recovery (100.09 ± 0.28 % and 99.98 ± 0.88 %; n=3) at 390nm and 530nm, respectively, reflected the method freedom from interferences.
Highlights
IntroductionKanamycin (Fig.1) is an aminoglycoside antibiotic obtained from the soil bacterium Streptomyces kanamyceticus, used parenterally in the treatment of various infections, especially those caused by gram-negative bacteria (Pestka, 1975)
The repeatability and reproducibility results showed a low relative standard deviation values (RSD % < 2), which reflected the precision of the developed method
Kanamycin (Fig.1) is an aminoglycoside antibiotic obtained from the soil bacterium Streptomyces kanamyceticus, used parenterally in the treatment of various infections, especially those caused by gram-negative bacteria (Pestka, 1975)
Summary
Kanamycin (Fig.1) is an aminoglycoside antibiotic obtained from the soil bacterium Streptomyces kanamyceticus, used parenterally in the treatment of various infections, especially those caused by gram-negative bacteria (Pestka, 1975). Several methods were reported for the analysis of KM in bulk form, pharmaceutical dosage form, and in biological fluids (Mahmoud et al, 2013; Mirela et al, 2007, Ahmed et al, 2007; Sekkat et al, 1989; Kim et al, 2001), these methods are either expensive, require many chemical reagents or sophisticated instruments. The aim of the present work was to develop simple and accurate colorimetric method for the determination of KM in bulk and pharmaceutical forms using ascorbic acid.
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