Abstract
Dendropanax morbifera Leveille (Araliaceae) has been used in traditional oriental remedies for cancer, inflammation, diabetes, and thrombosis. However, a validated analytical method, standardization, and optimization of extraction conditions with respect to biological activity have not been reported. In this study, a simple and validated HPLC method for identifying and quantifying active substances in D. morbifera was developed. Hot water and ethanolic D. morbifera leaf extracts from different production regions were prepared and evaluated with regard to their chemical compositions and biological activities. The contents of active compounds such as rutin and chlorogenic acid were determined in four samples collected from different regions. The 80% ethanolic extract showed the best antioxidant activity, phenolic content, reducing power, and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed the presence of chlorogenic acid and rutin in D. morbifera leaf extracts. The antioxidant and XO inhibitory activity of D. morbifera extract could be attributed to the marker compounds. Collectively, these results suggest that D. morbifera leaves could be beneficial for the treatment or prevention of hyperuricemia-related disease, and the validated HPLC method could be a useful tool for the quality control of food or drug formulations containing D. morbifera.
Highlights
Dendropanax morbifera Leveille is a subtropical evergreen tree which belongs to the familyAraliacea, and it has been used in traditional medicines for the treatment of infectious diseases, dermatopathy, and headaches [1]
We report the preparation of various hot water and ethanolic extracts of D. morbifera leaves collected from four different regions in Korea as well as the development of a simple and validated high-performance liquid chromatography (HPLC) method for chemical profiling and standardization of the plant extracts
The HPLC conditions were optimized for the mobile phase composition, column temperature, wavelength, and flow rate
Summary
Dendropanax morbifera Leveille is a subtropical evergreen tree which belongs to the family. Araliacea, and it has been used in traditional medicines for the treatment of infectious diseases, dermatopathy, and headaches [1]. Previous studies have reported that D. morbifera exhibit various pharmacological effects, including antioxidant [2], antidiabetic [3], hepatoprotective [4], anticomplementary [5], and antiatherogenic activities [6]. D. morbifera leaf has been traditionally used as a botanical remedy in Asia [7]. The efficacy of extracts and active constituents prepared with D. morbifera leaves as a medicinal source has been investigated in a few studies to date. Several research studies have reported various pharmacological activities of D. morbifera leaves and their. There has been a great effort to find candidates from natural products to effectively control metabolic disease [8]
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